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Table 1 Plasmodium vivax circumsporozoite gene variation in isolates from the central Pacific Ocean coast of Latin America

From: Molecular epidemiology of Plasmodium vivax in Latin America: polymorphism and evolutionary relationships of the circumsporozoite gene

Geographic origin

Total (n)

acsp CRR genotype:

Csp nucleotide sequence:

Type

n

PCR Product size (bp)

RFLP type

Isolate code

Date of collection

b, fGene sequence (bp)

# of CRRu

Mexico: Southern Chiapas: (batch 2002–2005)

2 s

vk210

1

~700

a

Mxch13

May 5, 2000

657 II

20

vk247

1

e>700

Mxch10

March 3, 1999

714

19

70

vk210

9 s

~700

a

Mxch1

Jul 5, 2005

c894 I

20

12 m

Mxch2

Oct 3, 2002

“

“

Mxch3

March 10, 2005

“

“

 

vk210

7 s

~700

b

Mxch4

July 4, 2002

c900

18

Mxch5

Feb 4, 2003

“

“

5 m

Mxch7

May 16, 2002

“

“

  

vk210

5 m

~500

c

Mxch3

-

c738

12

Mxch5

-

“

“

Mxch8

Nov 17, 2003

“

“

Mxch9

July 4, 2002

“

“

  

vk247

33 s

e>700

-

Mxch6

July 2, 2002

c951

19

20 m

Mxch7

-

“

“

Mxch8

-

“

“

Mxch9

-

“

“

Mxch12

Feb 16, 2004

“

“

Mxch11

Aug 16, 2004

714

“

d(batch 2006–2008)

vk210

(1 s)

~700

d

Mxch14

Nov 6, 2006

639

18

Oaxaca

2 s

vk247

 

e>700

-

MxO

Feb 22, 2007

714

19

Nicaragua

37 s

 

20

~700

a

Nir1

Jan 11, 2007

711 III

22

vk210

12

~650

e

Nichn

May 17, 2007

630

19

 

5

~600

f

Nir2,

Nov 27, 2006

603

18

Nichg

July 2, 2007

603

“

Peru

9 s

vk247

 

e>700

-

Peru1

May, 2008

c951

19

  1. s, single csp repeat genotype infection ; m, isolates with mixed vk210-vk247 or vk210-vk210 infections (in bold letters).
  2. aNested PCR (fragments amplified with oligonucleotides CSP-f1/CSP-r1 followed by primers CSP-f2/Pv9a).
  3. bOnly those sequences obtained from the amplified product obtained by the first set of primers (CSP-f1/CSP-r1).
  4. CRRu, central repeat units.
  5. c225 nucleotides from the amino end were also obtained for these isolates.
  6. dSee data of batch 2006–2008 in Table 2.
  7. eMolecular size slightly larger and discernible from vk210a fragment.
  8. fThree different nucleotide sequences (I, II and III) were detected for RFLP-vk210a.