A novel, single-amplification PCR targeting mitochondrial genome highly sensitive and specific in diagnosing malaria among returned travellers in Bergen, Norway

Table 1 Primers applied for the amplifications and sequencing examined in this study

Primer¹	Sequence	Published by
rPLU 6 forward	5^′-tta aaa ttg cag tta aaa cg	Snounou et al. [9]
rPLU 5 reverse	5^′-cct gtt gtt gcc tta aac ttc	Snounou et al. [9]
PgMt19 F3 forward	5^′-tcg ctt cta acg gtg aac	Polley et al. [21]
PgMt19 B3 reverse	5^′-aat tga tag tat cag cta tcc ata g	Polley et al. [21]
*Plasmodium falciparum* forward	5^′-aac aga cgg gta gtc atg att gag	Padley et al. [26]
*Plasmodium vivax* forward	5^′-gag cgt tca aag caa aca ga	This study
*Plasmodium ovale* forward	5^′-ctg ttc ttt gca ttc ctt atg c	Padley et al. [26]
*Plasmodium malariae* forward	5^′-cgt taa gaa taa acg cca agc g	Padley et al. [26]
Species-specific reverse	5^′-gta tct gat cgt ctt cac tcc c	Padley et al. [26]

ISSN: 1475-2875