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Table 1 Agreement between 18 s, modified cytochrome b and original cytochrome PCR assays

From: Combined DNA extraction and antibody elution from filter papers for the assessment of malaria transmission intensity in epidemiological studies

PCR assay

Filter paper number

Positivity, % (n/N)

Agreement, %

Kappa

Sensitivity, % (95% CI)

18S rRNA

Single

12.9 (31/240)

90.0

0.666

56.4 (42.3 - 69.7)

Double

16.7 (40/240)

93.8

0.804

72.7 (59.0 - 83.9)

Modified Cytochrome B

Single

15.4 (37/240)

92.5

0.760

67.3 (53.3 - 79.3)

Double

18.3 (44/240)

95.4

0.861

80.0 (67.0 - 89.6)

Original Cytochrome B

Single

17.9 (43/240)

95.0

0.847

78.2 (65.0 - 88.2)

Double

19.2 (46/240)

96.3

0.887

83.6 (71.2 - 92.2)

  1. The agreement, kappa value and sensitivity were calculated by comparing individual PCR conditions with ‘true positivity’ that was defined as positivity in any one of the PCR assay variants. The abbreviation n/N indicates PCR positive individuals (n) as a proportion of the total sample size (N).