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Table 1 Agreement between 18 s, modified cytochrome b and original cytochrome PCR assays

From: Combined DNA extraction and antibody elution from filter papers for the assessment of malaria transmission intensity in epidemiological studies

PCR assay Filter paper number Positivity, % (n/N) Agreement, % Kappa Sensitivity, % (95% CI)
18S rRNA Single 12.9 (31/240) 90.0 0.666 56.4 (42.3 - 69.7)
Double 16.7 (40/240) 93.8 0.804 72.7 (59.0 - 83.9)
Modified Cytochrome B Single 15.4 (37/240) 92.5 0.760 67.3 (53.3 - 79.3)
Double 18.3 (44/240) 95.4 0.861 80.0 (67.0 - 89.6)
Original Cytochrome B Single 17.9 (43/240) 95.0 0.847 78.2 (65.0 - 88.2)
Double 19.2 (46/240) 96.3 0.887 83.6 (71.2 - 92.2)
  1. The agreement, kappa value and sensitivity were calculated by comparing individual PCR conditions with ‘true positivity’ that was defined as positivity in any one of the PCR assay variants. The abbreviation n/N indicates PCR positive individuals (n) as a proportion of the total sample size (N).