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Table 2 Consistency of outcomes in different PCR assays in relation to the amount of filter paper material used for extraction

From: Combined DNA extraction and antibody elution from filter papers for the assessment of malaria transmission intensity in epidemiological studies

Filter paper number

Never positive (%)

Positive in 1/3 PCR assays (%)

Positive in 2/3 PCR assays (%)

Positive in 3/3 PCR assays (%)

Single

80.8 (194/240)

2.9 (7/240)

5.4 (13/240)

10.8 (26/240)

Double

79.6 (191/240)

1.3 (3/240)

4.6 (11/240)

14.6 (35/240)

  1. The proportion (n/N) of positive PCR assays when aliquots of the same extracted material was used in three different PCR assays.
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Malaria Journal

ISSN: 1475-2875

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