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Table 1 Published primers and probes used in analysis

From: Comparative evaluation of published real-time PCR assays for the detection of malaria following MIQE guidelines

Assay name

Primer and Probe sets as published in 5—3 orientation

Ref #

PLU3

F: GCTCTTTCTTGATTTCTTGGATG

[14]

R: AGCAGGTTAAGATCTCGTTCG

P: ATGGCCGTTTTTAGTTCGTG

MACH

F: ACATGGCTATGACGGGTAACG

[10]

R: TGCCTTCCTTAGATGTGGTAGCTA

P: TCAGGCTCCCTCTCCGGAATCGA

CYTB

F: TACTAACTTGTTATCCTCTATTCCAGTAGC

[13]

R: CCTTTAACATCAAGACTTAATAGATTTGGA

P: G + TGC + TAC + CAT + GTA + AAT + GTAA

WHO

F: CAGATGTCAGAGGTCAAATTCTAAGATT

[12]

R: TCCCTTAACTTTCGTTCTTGATTAATG

P: CTGGAGACGGACTACTGCGAAAGCATTTG

FAL

F: CTTTTGAGAGGTTTTGTTACTTTGAGTAA

[7]

R: TATTCCATGCTGTAGTATTCAAACACAA

P: TGTTCATAACAGACGGGTAGTCATGATTGAGTTCA

PLASMO

F: GTTAAGGGAGTGAAGACGATCAGA

[11]

R: AACCCAAAGACTTTGATTTCTCATAA

P: ACCGTCGTAATCTTAACCATAAACTATGCCGACTAG

TURBO

F: GTAATTGGAATGATAGGAATTTACAAGGT

[9]

R: TCAACTACGAACGTTTTAACTGCAAC

 
 

P: TGCCAGCAGCCGCGGTAATTC

 
  1. Primers and probes sequences as published. Forward and reverse primers are shown as F and R. All probes shown as P were labelled with 6-carboxy-fluorescein (FAM) as a reporter and 6-carboxy-tetramethylrhodamine (TAMRA) as a quencher to maintain uniformity. The last column shows the reference where primers and probe for each assay were obtained.