Table showing the number of Days (D) the samples became positive (P) by either microscopy (Micro) or qPCR assays. D1 is the day when the first qPCR assay was positive. The designation of days is arbitrary for comparison of qPCR assays to microscopy and does not reflect the actual day of qPCR positivity post infection challenge. DBM is the number of days samples were qPCR positive before they became microscopy positive. The actual Cq values are not shown. The last column shows the average number of days (Avg DBM) the samples were qPCR positive (for each assay) before they were microscopy positive for all the five volunteers.