Mice in Plasmodium berghei ANKA-mono-infection group and Schistosoma japonicum-P. berghei ANKA-co-infection group were compared at different time points according to data obtained by flow cytometry. Proportion and absolute number of CD4+CD25+Foxp3+Tregs, IL-10-secreting Tregs, and IFN-γ-secreting Tregs were analysed in A, B, C, D, E, F, respectively. Gating strategy for identifying splenic Tregs were shown on G and H by representative dot plots. At each time point, at least three mice per group were sacrificed. Bars represent the mean values ± SD. Student t test by comparisons between the two groups was performed with * indicating P<0.05 and # indicating P<0.01.