Indirect immunofluorescence microscopy with anti-rPvCSP sera on the P. vivax sporozoites. Mouse antiserum to rPvCSP was incubated with smears of VK210 sporozoites (VK210) and of VK247 sporozoites (VK247). In both VK210 and VK247 sporozoites, the anti-rPvCSP serum reactivity was shown in green and nuclear staining with DAPI was shown in blue (right panels). Bright field DIC images were presented in the left panels. Black bar indicates 5 μm.