Association of Plasmodium SmD1 protein with parasite protein arginine methyltransferase 5 (PRMT5). (A) Amino acid sequence alignment of Plasmodium PRMTs methyltransferase motifs with corresponding Trypanosoma and human homologues. (B) Co-localization of PfSmD1 and PfPRMT5 proteins. Immunofluorescence assay was performed using mouse anti-PfSmD1 and rat anti-PfPRMT5 antisera (1:200 dilution) on thin blood smears of P. falciparum. (C) Immunoprecipitation of parasite proteins with mouse anti-PfSmD1 serum or control mouse pre-immune serum and probing with rat anti-PfPRMT5 antibodies in an immunoblot assay. Input shows the 25% of total parasite proteins used in an immunoprecipitation assay. Arrow indicates the precipitated PfPRMT5 protein band.