Figure 3
From: Annotation and characterization of the Plasmodium vivax rhoptry neck protein 4 (Pv RON4)
Pv RON4 expression in VCG-1 schizonts. (A) Schematic representation of Pv RON4. The most relevant characteristics in the protein’s primary sequence are shown. Red shows signal peptide location, blue a coiled-coil α-helical motif and grey the tandem repeats. Red lines indicate conserved cysteines between Sal-1, VCG-1, Mauritania I and India VII strains. Inoculated peptides’ localization and sequence are shown in the boxes. (B) Pv RON4 recognition in schizont lysate. Lane 1: pre-immune serum; lane 2: immune serum; lane 3: immune serum pre-incubated with inoculated peptides. (C) Immunofluorescence studies on schizonts. The arrows indicate the presence of the protein (red) and the apical pattern suggesting its localization in the apical organelles. Blue shows schizont nuclei stained with DAPI.