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Figure 1 | Malaria Journal

Figure 1

From: An integrated strategy for efficient vector construction and multi-gene expression in Plasmodium falciparum

Figure 1

Schematic of the homology-based (yeast HR and Gibson assembly) and traditional restriction/ligation cloning strategies selected as part of an integrated framework for the orthogonal assembly of Plasmodium falciparum constructs. Beginning with a common primer set, PCR products and the desired vector backbone (see Figure 2 for details), the identical target plasmid can be assembled using any of these approaches individually or in parallel.

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