The Thosea asigna virus 2A-like peptide (T2A) enables expression of two functional proteins in Plasmodium falciparum from a single expression cassette . (A) Schematic of FL-nptII and control constructs. (B) Both T2A- and T2Am- containing constructs produce active FL. (C) Western blot detection of FL- and nptII- containing proteins. (D) Northern blot analysis of FL- containing transcripts in transfected parasites. 3D7 + FL indicates the inclusion of a synthetic FL mRNA produced by in vitro transcription.