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Figure 1 | Malaria Journal

Figure 1

From: Ex vivo tetramer staining and cell surface phenotyping for early activation markers CD38 and HLA-DR to enumerate and characterize malaria antigen-specific CD8+ T-cells induced in human volunteers immunized with a Plasmodium falciparum adenovirus-vectored malaria vaccine expressing AMA1

Figure 1

Gating scheme. PBMC were obtained from volunteers vaccinated with the NMRC-M3V-Ad-PfCA Vaccine (AdCA) and then CD8+ T cells were enriched by magnetic selection and labelled with cognate tetramers. Subsequently, cells were surfaced labelled using a single panel of antibodies for CD3+ T cells, CD8+ T cells, CD38+ cells and HLA-DR+ cells and then acquired on an LSR II flow cytometer. Data were analysed by Flow Jo and cells were gated as shown.

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Malaria Journal

ISSN: 1475-2875

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