Figure 1

Gating scheme. PBMC were obtained from volunteers vaccinated with the NMRC-M3V-Ad-PfCA Vaccine (AdCA) and then CD8+ T cells were enriched by magnetic selection and labelled with cognate tetramers. Subsequently, cells were surfaced labelled using a single panel of antibodies for CD3+ T cells, CD8+ T cells, CD38+ cells and HLA-DR+ cells and then acquired on an LSR II flow cytometer. Data were analysed by Flow Jo and cells were gated as shown.