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Figure 2 | Malaria Journal

Figure 2

From: Ex vivo tetramer staining and cell surface phenotyping for early activation markers CD38 and HLA-DR to enumerate and characterize malaria antigen-specific CD8+ T-cells induced in human volunteers immunized with a Plasmodium falciparum adenovirus-vectored malaria vaccine expressing AMA1

Figure 2

Identification ex vivo of malaria antigen epitope-specific CD8+T cells by cognate tetramer staining. PBMC were obtained at pre {bleed 1}- and post {bleed 2 (day 22/23)}-vaccination. Enriched malaria antigen epitope-specific CD8+ T cells were labelled with cognate tetramers TLDEMRHFY:HLA-A*01:01(Vol. 126 and 184) and NEVVVKEEY:HLA-B*18:01(Vol. 128), surface labelled and then analysed as shown in Figure 1.

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Malaria Journal

ISSN: 1475-2875

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