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Table 1 AMA1-based tetramers used in this study

From: Ex vivo tetramer staining and cell surface phenotyping for early activation markers CD38 and HLA-DR to enumerate and characterize malaria antigen-specific CD8+ T-cells induced in human volunteers immunized with a Plasmodium falciparum adenovirus-vectored malaria vaccine expressing AMA1

  

Tetramer tested

   

Volunteer

Volunteer HLA

Tetramer HLA

AMA-1 epitope

IC50nM1

AMA1 pool

sfc/mill to pool4

119

A*30:022

A*01:01

TLDEMRHFY

36

Ap4

266

  

A*30:02

RYKSHGKGY3

201

Ap8

547

125

A*02:01

A*02:01

TQKCEIFNV

658

Ap8

81

126

A*01:01

A*01:01

TLDEMRHFY

17

Ap4

228

  

A*30:02

RYKSHGKGY

172

Ap8

153

128

A*02:01

A*02:01

TQKCEIFNV

658

Ap8

31

 

B*18:01

B*18:01

NEVVVKEEY

12

Ap10

215

  

B*18:01

YEYPLHQEH

26

Ap1

71

  

B*18:01

NEFPAIDLF

9

Ap7

99

147

A*30:01

A*30:02

RYKSHGKGY

172

Ap8

13

184

A*01:01

A*01:01

TLDEMRHFY

17

Ap4

568

  1. 1IC50 nM values for the volunteer’s HLA type were predicted using NetMHC web-based software [14].
  2. 2A*30:02 is a member of the A01 supertype.
  3. 3The predicted epitope = HGKGYNWGN.
  4. 4ELISpot response to parent AMA1 peptide pool containing the epitope.