Identification and characterization of the merozoite surface protein 1 (msp1) gene in a host-generalist avian malaria parasite, Plasmodium relictum (lineages SGS1 and GRW4) with the use of blood transcriptome

Table 1 Amino acid similarity (%) between different Plasmodium species/lineages

Block	X	Pg/SGS1	Pf/SGS1	Pg/Pf	SGS1/GRW4	Pf amino acid positions	Pg amino acid positions
All		49.8	32.9	33.7	89.9	whole	whole
1	c	44.4	34.1**	46.4	ND	1–55	1–56
2	r	-	-	-	-	56–111	-
3	c	62.9	41.3	42.7	84.7*	112–313	57–255
4	v	39.1	28.1	21.9	86.4	314–345	256–277
5	c	62.9	60	74.3	97.1	346–380	278–312
6	v	44.9	25.8	25.4	93.7	381–609	313–536
7	sc	55.4	48.6	50	93.2	610–682	537–610
8	V¹	36.1	14.4	16.3	84.7	683–779	611–717
9	sc	62.6	49.5	52.3	96.3	780–886	718–824
10	v	32.6	17.4	19.7	88.3	887–1024	825–953
11	sc	54.3	48.6	42.9	91.4	1,025–1,059	954–988
12	c	57.5	51.2	50.6	97.5	1,060–1,138	989–1,067
13	sc	52.2	45.2	39.3	96.4	1,139–1,222	1,068–1,149
14	v	20.8	14.9	12.7	76.1	1,223–1,288	1,150–1,220
15	c	44.6	42.2	37.1	89	1,289–1,316	1,221–1,308
16	v	54.8	27.9	29.1	98*	1,317–1,602	1,309–1,528
17	c	66	51.5	50.5	ND	1,603–1,702	1,529–1,622

Plasmodiun gallinaceum (Pg), Plasmodium relictum lineage SGS1 (SGS1), Plasmodium relictum lineage GRW4 (GRW4), Plasmodium falciparum (Pf) Plasmodium yoelii (Py).
Row X shows whether a block had been described previously as conserved (c), including repetitive motifs (r), variable (v) or semi-conserved (sc). The positions of the blocks are described in the last two columns after aligning P. falciparum (X05624.2) and P. gallinaceum (AJ809338.1).
**only partial sequence of SGS1, *only partial sequence of GRW4, ND: No sequence of the block in GRW4, - block is lacking for SGS1, GRW4 and Pg. Blocks were assigned according to Tanabe et al. [16]. ¹According to Rich and Ayala this block is also containing repetitive sections [22].

ISSN: 1475-2875