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Figure 2 | Malaria Journal

Figure 2

From: An alternative protocol for Plasmodium falciparum culture synchronization and a new method for synchrony confirmation

Figure 2

Synchrony confirmation of samples from Percoll synchronization method. The synchrony of two S2 aliquots (3- to 6-hr, see Additional file 1) was confirmed by incubating the RS samples in complete media until they reached SS. The 0-hr denotes the calculated start of egress/invasion. The matured SS parasites were isolated over a 20%/60% Percoll cushion at the calculated egress time (S2-2) or 3-hr before the calculated egress time (S2-1) and re-suspended in complete media containing 1% RBC. Aliquots were taken (0.5 ml) at the indicated time points and ran over a 70% Percoll cushion, washed, and frozen. Samples were later re-suspended to a 1% haematocrit using resuspension buffer (0.5 ml) and 0.1 ml aliquots were assayed in triplicate using the SyBr Green assay format.

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