Human genetic polymorphisms in the Knops blood group are not associated with a protective advantage against Plasmodium falciparum malaria in Southern Ghana

Table 1 Primer sequences and conditions for the PCR and ligase detection reaction (LDR)

Primers	Microsphere	Sequences
		Fw 5’-TCTCTCAGTCATATCTTGTG-3’
PCR		Rw 5’-CTATTATGTTTATGCCAATTAA-3’
Conditions		95°C 15 min, 40 cycles: 95°C 30 sec, 52°C 30 sec, 62°C 60 sec, 72°C 10 min
McC ^a	039	5’-acaaatatctaactactatcacaaTCGGTGTATTTCTACTAATA-3’
McC ^b	055	5’-acatcaaattctttcaatatcttcCGGTGTATTTCTACTAATG-3’
McC common		5’-PHOS-AATGCACAGCTCCAGAAGTT-BIO-3’
Sl1	076	5’-tctcatctatcatactaattctttAGAAGTTGAAAATGCAATTA-3’
Sl2	095	5’-ttctttattctcattatcacatcaAGAAGTTGAAAATGCAATTG-3’
Sl common		5’-PHOS-GAGTACCAGGAAACAGGAGT-BIO-3’
Kn ^a	022	5’-caaacaaacattcaaatatcaatcAGAACAGCTGTTTGAGCTTG-3’
Kn ^b	037	5’-tacaacatctcattaacatatacaGAACAGCTGTTTGAGCTTA-3’
Kn common		5’-PHOS-TGGGAGAACGGTCAATATAT-BIO-3’
KCAM ⁺	057	5’-acttacaataactactaatactctCTTTACCCTCACTGAGATCA-3’
KCAM ^-	078	5’-tttacaaatctaatcacactatacCTTTACCCTCACTGAGAT-3’
KCAM common		5’-PHOS-TCAGATTTAGATGTCAGCCC-BIO-3’
LDR Conditions		95°C 1 min, 32 cycles: 95°C 15 sec, 60°C 2 min

Primer sequences for the CR1 PCR and LDR conditions. The LDR (ligase-detection reaction) primers consist of set of three for each loci; two allele specific primers with anti-TAGs (small letters) matching the TAG sequence on the microsphere (Luminex xTAG microsphere number shown here), and one common primer marked with phosphate (PHOS) at the 5’ end and biotin (BIO) at the 3’ end. The site of the single nucleotide polymorphisms are highlighted and underscored.

ISSN: 1475-2875