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Table 2 List of the controls used in each PCR assays for the validation of DNA extraction and/or the real-time PCR runs

From: An innovative tool for moving malaria PCR detection of parasite reservoir into the field

QC name Number used in 96-well plates Type Concentration
PCR HPC PCR High Positive Control 1 Pf 3D7 DNA extract (QiaAmp DNA mini kit) 0.1 ng/μl
PCR LPC PCR Low Positive Control 1 Pf 3D7 DNA Extract (QiaAmp DNA mini kit) 0.001 ng/μl
PCR NC PCR Negative Control 2 H2O N/A
Ext HPC Extraction High Positive Control 2 Dried blood spot (4mmØ ) with Pf 3D7 blood 500 parasites/μl
Ext LPC Extraction Low Positive Control 2 Dried blood spot (4mmØ ) with Pf 3D7 blood 5 parasites/μl
Ext NC Extraction Negative control 2 Dried blood spot (4mmØ ) with Pf negative blood No parasite
Pf P. falciparum Positive Control 1 Plasmid (Pf 400 bp insert) 0.001 ng/μl
Pv P. vivax Positive Control 1 Plasmid (Pv 400 bp insert) 0.001 ng/μl
Pm P. malariae Positive Control 1 Plasmid (Pm 400 bp insert) 0.001 ng/μl
Po P. ovale Positive Control 1 Plasmid (Po 400 bo insert) 0.001 ng/μl