Skip to main content

Advertisement

Table 3 Threshold of detection of the real-time PCR screening (including sample volume analysed, DNA extraction and real-time PCR screening): percentage of positive results according to the dilution of parasites

From: An innovative tool for moving malaria PCR detection of parasite reservoir into the field

Dilution (parasites/μl) % of positive results (No positives/No tested)
  Assay 1 Assay 2 Average
1,000 100% (8/8) 100% (8/8) 100% (16/16)
100 100% (8/8) 100% (8/8) 100% (16/16)
10 100% (8/8) 100% (8/8) 100% (16/16)
5 ND 100% (8/8) 100% (8/8)
2 ND 100% (8/8) 100% (8/8)
1 87% (7/8) 75% (6/8) 81% (13/16)
0.1 0% (0/8) 0% (0/8) 0% (0/8)
0.01 0% (0/8) ND 0% (0/8)