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Table 5 Performances of the real-time screening PCR and the genus-specific 18S rRNA nested PCR: according to Ct range

From: An innovative tool for moving malaria PCR detection of parasite reservoir into the field

Real-time screening PCR Ct range Number of positive samples by using
  Real-time screening PCR Genus-specific18S rRNA nested PCR (% of concordance)
16 to 29 cycles 34 34 (100%)
30 to 35 cycles 10 5 (50%)
> 35 cycles 10 0 (0%)