Script analysis and performance of late stage gametocyte assay. A. Top panel no compound treatment. Bottom panel 5 μM puromycin, 72 hour treated late stage gametocytes. MTR channel mask, GFP channel Mask, analysed Gametocyte Mask and MTR-GFP overlay of Opera acquired images. B. Linearity graph- gametocyte number per well against script identified viable gametocytes per well. C. Opera acquired image of gametocytes after 72 hours incubation and MTR staining. A X60 water objective was used to capture detailed images. Classical stage V gametocytes are observed.