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Figure 5 | Malaria Journal

Figure 5

From: Cytoplasmic free Ca2+ is essential for multiple steps in malaria parasite egress from infected erythrocytes

Figure 5

A23187-induced and Ca2+-dependent parasite death. A. Immature schizonts from infected erythrocytes critically swelled with extruded parasitophorous vacuoles. Cultures were treated with 10 μM A23187 in chambers for two hours in the presence of fluorescent antibodies to the major erythrocyte surface protein glycophorin A (red colour in upper images) and with fluorescent phalloidin (green colour in lower images) to detect the major erythrocyte cytoskeletal protein F-actin. Note the unlabelled membranes of the parasitophorous vacuoles (black arrowheads) extruded from the labelled erythrocyte membranes; bar = 5 μm. B. Extended treatment (2 hours) of cultures with increased concentrations of ionophore inhibited parasite egress and led to accumulation of damaged schizonts (a representative experiment, mean of three measurements, total number of infected cells analysed, 1,402).

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