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Table 1 Data overview of IC50 speed assay and stage specificity analysis

From: Fast in vitro methods to determine the speed of action and the stage-specificity of anti-malarials in Plasmodium falciparum

Compound

Assay 1 (A1):

Conclusion A1

Assay 2 (A2):

Conclusion A2

Concluded speed of action

 

IC50 speed assay

 

Stage specificity analysis

  
 

(ratio of IC50 24 h/IC50 72 h)a

 

(at 6-100x IC50)

  

Chloroquine

1.1

Fast

>95% action on Rb

Fast (cidal)

Fast (cidal)

   

>95% action on Sb

  

Artesunate

1.1

Fast

>95% action on R

Fast (cidal)

Fast (cidal)

   

>95% action on S

  

Atovaquone

3.6

Not fast

~50-70% action on R

Not fast (cidal)

Not fast (cidal)

   

~75-90% action on S

  

Pyrimethamine

8.3

Not fast

~5% action on R

Not fast (cidal)

Not fast (cidal)

   

~90% action on S

  

1

4.3

Not fast

~0-75% action on R

Not fast (cidal)

Not fast (cidal)

   

>95% action on S

  

2

1.6

Fast/Not fast

~40- >95% action on R

Not fast (cidal)

Not fast

   

>95% action on S

  

3

1.2

Fast

>95% action on R

Fast (cidal)

Fast (cidal)

   

>95% action on S

  
  1. aRatios are means from ≥ 3 independent experiments performed with P. falciparum strain NF54.
  2. bR and S are abbreviations for the ring and schizont stages, respectively.