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Table 1 Oligonucleotides used for NASBA, hybridization and in vitro RNA

From: Evaluation of Plasmodium falciparum gametocyte detection in different patient material

Oligonucleotides Sequence 5´ to 3´
Pfs16.F WT 5‘-AGT TCT TCA GGT GCC TCT CTT CA-3‘
Pfs16.R WT 5’-T7-AGC TAG CTG AGT TTC TAA AGG GCA-3’
Pfs16.F Q 5’-CAA CAT GAA TAT TCG AAA GTT CAT ACC-3’
Pfs16.R Q 5’-AGA ATC ATC TCC TTC GTC TTC TTC-3’
Pfs16 probe 5’-6-FAM-CGATCG-GCT GTT GGA CCT AAT CTA ATC TAG GTG GA-CGATCG-Dabsyl-3‘
Pfs25.F WT 5‘-GAC TGT AAA TAA ACC ATG TGG AGA-3‘
Pfs25.R WT 5‘-T7-CAT TTA CCG TTA CCA CAA GTT A-3‘
Pfs25.F Q 5’-GAA TTC GAC TGT AAA TAA ACC ATG TGG AGA-3’
Pfs25.R Q 5’-AAG CTT CAT TTA CCG TTA CCA CAA GTT A-3’
Pfs25 probe 5‘-Texas Red-CGATCG-CCC GTT TCA TAC GCT TGT AA-CGATCG –Dabsyl-3’
18S.F WT 5’-GTC ATC TTT CGA GGT GAC TT-3’
18S.R WT 5’-AAC TTT CTC GCT TGC GCG AA-3’
18S probe 5’-6-FAM-CGATCG-GAG AAA TCA AAG TCT TTG GG-CGATCG-Dabsyl-3’
M13.F 5'-GTA AAA CGA CGG CCA GT-3'
M13.R 5'-CAG GAA ACA GCT ATG AC-3'