Figure 3
Changes in prevalence of known drug resistance-associated mutations over time. Prevalence of resistance-associated mutations in pfcrt (A) and pfmdr1 (B) with corresponding 95% point-wise confidence intervals. Mutations were measured by high-resolution melt (HRM) technology and prevalence was calculated by dividing the number of samples containing at least one mutant allele by the total number of samples genotyped each year. Asterisks indicate significant changes over time (P < 0.05 by Fisher-Hamilton exact test).