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Table 3 Associations between wild-type and mutant genotypes and ex vivo drug responses

From: Changes in drug sensitivity and anti-malarial drug resistance mutations over time among Plasmodium falciparum parasites in Senegal

  Median IC50Values
(Interquartile Range)
  Amodiaquine Artemisinin Chloroquine Mefloquine
Allele Wild-type Mutant P Wild-type Mutant P Wild-type Mutant P Wild-type Mutant P
 N(wt)/N(mut)             
pfcrt 72-76a 9 12 0.004 9 7 0.005 14 99 < 0.0001 42 36 0.3
 160/182 (6,15) (8,19) (6,15) (4,10) (11,28) (53,196) (21,57) (21,52)
pfcrt A220S 9 12 0.0005 9 7 0.01 14 99 < 0.0001 42 36 0.3
 163/174 (6,14) (8,19) (6,15) (4,10) (11,28) (53,196) (21,57) (21,52)
pfcrt N326S 10 15 0.01 8 9 0.6 28 139 < 0.0001 38 38 0.7
 295/40 (7,16) (9,20) (5,13) (5,12) (13,93) (84,217) (21,54) (28,56)
pfmdr1 N86Y 10 9 0.6 8 5 0.0002 33 75 0.4 41 17 < 0.0001
 296/37 (7,16) (6,17) (5,13) (3,8) (14,101) (12,201) (26,57) (9,22)
pfmdr1 Y184F 10 11 0.1 7 8 0.01 29 34 0.6 41 36 0.3
 136/202 (6,16) (7,16) (4,11) (6,14) (12,109) (13,114) (21,58) (21,52)
pfmdr1 N1042D 10 8 0.1 8 4 <0.0001 33 47 1.0 38 38 0.9
 307/31 (7,16) (5,16) (6,13) (2,5) (13,109) (15,115) (21,53) (18,59)
  1. IC50 values are in nM. N(wt) = number of samples possessing only the wild-type allele. N(mut) = number of samples possessing at least one mutant allele. Mixed genotypes were detected at the following prevalence: pfcrt 72-76 (n = 22); pfcrt A220S (n = 19); pfcrt N326S (n = 4); pfmdr1 N86Y (n = 7); pfmdr1 Y184F (n = 22); pfmdr1 N1042D (n = 1). Genotypes were determined by high-resolution melt (HRM) genotyping. P-values were calculated using the Wilcoxon rank-sum test, and associations where P < 0.05 are shown in bold.
  2. apfcrt protein positions 72-76 were all perfectly correlated and were analysed as a haplotype rather than individually.