produced in Escherichia coli expression system and purified with ProBondTMpurification system. The pkMSP-142 with a size of ~42 kDa (arrows) was detected in (A) Coomassie brilliant blue stained SDS gel, and (B) Western blot probed with anti-XpressTM antibody. (A) Lanes 1 and 2, pRSET A at 0 and 2 hour respectively; lanes 3 and 4, purified pRSET A; lanes 6 and 7, recombinant pkMSP-142 at 0 and 2 hour respectively; lane 8 and 9, purified pkMSP-142. (B) Lane 1, purified pRSET A; lane 3, purified pkMSP-142. (C) Lanes 2 and 3, purified pkMSP-142 under reducing condition with sample boiling and without boiling respectively; lanes 4 and 5, purified pkMSP-142 under non-reducing condition with sample boiling and without boiling respectively. Lane 5 in (A), lane 2 in (B) and lane 1 in (C) were Prestained Broad Range Protein Marker.