Table 1 Primer and probe sequences
RT-PCR | Forward primer (plasmo1) | Reverse primer (plasmo2) | Probes |
|---|---|---|---|
Pfal | 5′-CTAGGTGTTGGATG-3′ | 5′-TTATGAGAAATCAAAGTCTTTGGGTT-3′ | 5′-FAM-AGCAATCTAAAAGTCACCTCGAAAGATGACT-DQ-3′ |
Pova | 5′-CGACTAGGTTTTGGATG-3′ | 5′-VIC-CGAAAGGAATTTTCTTATT-DQ-3′ | |
Pviv | 5′-GACTAGGCTTTGGATG-3′ | 5′-VIC-AGCAATCTAAGAATAAACTCCGAAGAGAAAATTCT-DQ-3′ | |
Pmal | 5′-GACTAGGTGTTGGATG-3′ | 5′-FAM-CTATCTAAAAGAAACACTCAT-DQ-3′ | |
PCR/genotyping | Forward primer | Reverse primer | |
Pfcrt | 5′-GTTCTTGTCTTGGTAAATGTGCTCA-3′ | 5′-CAATT TTGTTTAAAGTTCTTTTAGCAA-3′ |