Figure 2
From: Evaluation of bioluminescence-based assays of anti-malarial drug activity
Absolute and time-dependent decay of luminogenic substrates. Trophozoite stage Dd2luc (2% parasitaemia, 2% HCT) were lysed and an equal volume of the indicated (see key) luminogenic substrate added. (A) The decay in absolute mean value of RLU ± stdev (n = 5) for each luminogenic substrate (see key) is plotted over 100 minutes. (B) The relative decay for each luminogenic substrate is reported by plotting the mean RLU ± stdev as a proportion of the mean RLU at t = 0 mins.