Figure 3From: Evaluation of bioluminescence-based assays of anti-malarial drug activityThe effect of luminogenic substrate choice on the determination of IC 50 values. Trophozoite stage Dd2luc (2% parasitaemia, 2% HCT) were incubated for 48 hrs in the presence of the indicated concentration of drug prior to measurement of the absolute mean RLU ± stdev (n = 3) using (A) chloroquine and (C) artemether. Normalizing these data as a fraction of the controls (% growth) allows a dose–response curve to be plotted for each luminogenic substrate for (B) chloroquine and (D) artemether. The IC50 values determined for each luminogenic substrate (see key in Figure2) are shown.Back to article page