Table 1 Comparison of assay parameters from bioluminescence and fluorescence-based assays
From: Evaluation of bioluminescence-based assays of anti-malarial drug activity
Assay reagent | Z’score | Signal/noise ratio | %CVmax | %CVmin |
|---|---|---|---|---|
Bright-Glo | 0.74–0.84 | 15667–17746 | 5.2–6.7 | 14.8–16.2 |
SL substrate1 | 0.73–0.81 | 12853–13609 | 8.0–8.9 | 15.5–17.0 |
One-Glo | 0.77–0.86 | 8936–10030 | 4.7–6.3 | 14.3–19.5 |
Steady-Glo | 0.83–0.86 | 2063–2344 | 5.6–7.1 | 17.2–18.6 |
MSF | 0.72–0.79 | 4.30–4.39 | 4.0–6.6 | 2.8–5.8 |