Figure 2

PF3D7_1363700 transcripts are present in various stages of Plasmodium falciparum. PF3D7_1363700 transcripts are present in asexual blood stages, gametocytes, oocyst sporozoites, and salivary gland sporozoites as determined by reverse-transcription PCR (RT-PCR). cDNA was produced from RNA isolated from mixed erythrocytic stages (ES), enriched gametocytes, salivary gland sporozoites, oocyst sporozoites, and axenic liver stages. PCR amplifications were performed using PF3D7_1363700 specific primers and parasite control primers (+); P. falciparum merozoite surface protein 1 (MSP1) control primers for blood stage parasites, Pfs230 control primers for gametocytes, P. falciparum circumsporozoite (CS) primers for salivary gland and oocyst sporozoites and P. falciparum heat shock protein 70 (PfHSP70) primers for axenic liver stages. Plasmodium falciparum genomic DNA (gDNA) was used as a positive control while a no reverse transcriptase (−) reaction showed that there was no genomic DNA contamination. DNA size markers in base pairs (bp) are shown to the right of the figure.