Figure 6

PF3D7_1363700-GFP expression in various parasite stages. The PF3D7_1363700-GFP fusion protein of clonal population 81 (PfMGFP) was expressed only during the late asexual blood stages such as the trophozoites and schizonts. No expression was observed during the ring stage (data not shown), gametocyte stage and mosquito parasite stages (i.e., zygote, oocyst sporozoite, haemolymph sporozoite (HS), and salivary gland sporozoite (SGS). Expression of the fusion protein was under the control of the PF3D7_1363700 promoter. DAPI was used for parasite nuclei staining. Orange arrowheads denote the presence of parasites in the merged images. 3D7GFP merge images depict constitutively expressing parasites that were used as a positive control and –GFP merge are images of wild-type parasites that were used as a negative control. Fluorescent images shown are representative of PF3D7_1363700 expression during that specific stage and three biological replicates were performed for each stage observed.