Table 1 Primers based on the 18S rRNA gene in malaria parasites for nested PCR diagnosis of malaria infections
From: Performance of two rapid diagnostic tests for malaria diagnosis at the China-Myanmar border area
Species | Primer | Sequence (5’-3’) | Size of PCR product (bp) |
|---|---|---|---|
Plasmodium sp. | rPLU5 | CCTGTTGTTGCCTTAAACTTC | 1100 |
| Â | rPLU6 | TTAAAATTGTTGCAGTTAAAACG | Â |
P. falciparum | rFAL1 | TTAAACTGGTTTGGGAAAACCAAATATATT | 205 |
| Â | rFAL2 | ACACAATGAACTCAATCATGACTACCCGTC | Â |
P. vivax | PV18SF | GAATTTTCTCTTCGGAGTTTATTC | 419 |
| Â | PV18SR | GTAGAAAAGGGAAAGGGAAACTGTTA | Â |
P. malariae | PM18SF | GAGACATTCATATATATGAGTGTTTCT | 423 |
| Â | PM18SR | GGGAAAAGAACGTTTTTATTAAAAAAAAC | Â |
P. ovale | PO18SF | GAAAATTCCTTTTGGAAATTTCTTAG | 410 |
| Â | PO18SR | GGGAAAAGGACACTATAATGTATC | Â |
P. knowlesi | PK18SF | GAGTTTTTCTTTTCTCTCCGGAG | 424 |
| Â | PK18SR | GGGAAAGGAATCACATTTAACGT | Â |