Figure 2

PCR titration of genomic DNA using the genus specific primers. A) The schematic outlines the strategy for UCP detection. B) UCP detection of digoxigenin-biotin labelled amplicon generated from a serial dilution of P. falciparum DNA using semi-nested PCR. The equivalent # parasites per reaction were 38084, 3408, 1704, 852, 426, 213, 107, 53, 27, 13, 7, and 3, which corresponds to Lane numbers 1 through 12 on the image of the agarose gel. The insert is the ethidium bromide stained agarose gel (2%), which confirms the resulting amplicon products as a function of the serial dilution of Plasmodium falciparum DNA. Each lane contained 18 μl of the PCR product. The intensity of the phosphorescent UCP reporter particles was measured through excitation at 980 nm and emission at 455 nm.