Figure 3From: Improved assay to detect Plasmodium falciparum using an uninterrupted, semi-nested PCR and quantitative lateral flow analysisUCP detection analysis of clinical malaria saliva samples using the semi-nested asymmetric PCR protocol. UCP lateral flow assay: UCP reporter was used to detect the biotin-digoxigenin labelled amplicons generated using the single-step PCR amplification protocol. Parasite concentrations were determined using thick film microscopy of subject blood. The threshold is indicated as a horizontal dashed line.Back to article page