Table 1 Primer pairs to amplify DBL2β-C2 domain
From: Diversity of the var gene family of Indonesian Plasmodium falciparum isolates
Primer | Forward | Reverse |
|---|---|---|
Primers to amplify the whole DBL2β-C2 domain | ||
DBLβF-R | 5′-AGT GTG TTG AAG GAC GTA TGT-3′ | 5′-CCA AAC ATA TAT CTC TAT AAT CTC C-3′ |
Primers to amplify the inner sequences of DBL2β-C2 domain (primer walking) | ||
Pap1_DBLβ | 5′-ATG ACT GAA TGG GC(C/A) GAA TG-3′ | 5′-CAA GAA GTC ATA CAC GGA T-3′ |
Pap2_DBLβ | 5′-ATG ACT GAA TGG GC(C/A) GAA TG-3′ | 5′-TAC ATT CTG GAT CCT CTT C-3′ |
Pap3.1_DBLβ | 5′-ATG ACT GAA TGG GC(C/A) GAA TG-3′ | 5′-CGTTGACTTGTGTACCACCA-3′ |
Pap3.2_DBLβ | 5′-ATG ACT GAA TGG GC(C/A) GAA TG-3′ | 5′-ATG CGT CCT TAT ACT CTG G-3′ |
Kal1_DBLβ | 5′-ATG ACT GAA TGG GC(C/A) GAA TG-3′ | 5′-TAG TAC CAC CGA TTG AGC GT-3′ |
Kal2_DBLβ | 5′-ATG ACT GAA TGG GC(C/A) GAA TG-3′ | 5′-TGT TCA TCG TCT TCA CCT T-3′ |
Kal3_DBLβ | 5′-ATG ACT GAA TGG GC(C/A) GAA TG-3′ | 5′-TAG TAC CAC CGA TTG AGC GT-3′ |
Kal4_DBLβ | 5′-ATG ACT GAA TGG GC(C/A) GAA TG-3′ | 5′-TAG TAC CAC CGA TTG AGC GT-3′ |
Kal5_DBLβ | 5′-ATG ACT GAA TGG GC(C/A) GAA TG-3′ | 5′-TAG TAC CAC CGA TTG AGC GT-3′ |