Skip to main content

Table 1 Primers used for the construction of truncated pf JRTS mutants

From: Insights into the role of the junctional region of Plasmodium falciparum dihydrofolate reductase-thymidylate synthase

Name

Length (bases)

Sequence (5′ → 3′)

Utilities and description

JRTSΔ232-235

28

AAAGAATCCC ATG G AACAAAATTGTATA

Sense strand PCR primer for the construction of pET-pf JRTSΔ232-235. Bold-face letters represent Met introduced in front of Glu236. Underlined sequence is the restriction site for Nco I.

JRTSΔ232-251

28

AAAGAATCCC ATGG AA AAGAATGATGAC

Sense strand PCR primer for the construction of pET-pf JRTSΔ232-251. Bold-face letters represent Met-Glu introduced infront of Lys252. Underlined sequence is the restriction site for Nco I.

JRTSΔ232-265

28

AAAGAATCCC ATG G AATTTTACAAAAAT

Sense strand PCR primer for the construction of pET-pf JRTSΔ232-265. Bold-face letters represent Met introduced in front of Glu266. Underlined sequence is the restriction site for Nco I.

JRTSΔ232-271

28

AAAGAATCCC ATG G ACAAATATAAAATT

Sense strand PCR primer for the construction of pET-pf JRTSΔ232-271. Bold-face letters represent Met introduced in front of Asp272. Underlined sequence is the restriction site for Nco I

JRTSΔ232-274

57

CCCCTCTAGA AATAATTTTGTTTAACTTTAAGAAGGAGATATACCATG AAAATTAAT

Sense strand PCR primer for theconstruction of pET-pf JRTSΔ232-274. Bold-face letters represent Met introduced in front of Lys275. Underlined sequence is the restriction site for Xba I

JRTSΔ232-276

57

CCCCTCTAGA AATAATTTTGTTTAACTTTAAGAAGGAGATATACCATG AATTATGAA

Sense strand PCR primer for the construction of pET-pf JRTSΔ232-276. Bold-face letters represent Met introduced in front of Asn277. Underlined sequence is the restriction site for Xba I

JRTSΔ232-277

57

CCCCTCTAGA AATAATTTTGTTTAACTTTAAGAAGGAGATATACCATG TATGAAAAT

Sense strand PCR primer to prepare construct pET-pf JRTSΔ232-277. Bold-face letters represent Met introduced in front of Tyr278. Underlined sequence is the restriction site for Xba I

JRTSΔ232-299

28

AAAGAATCCC ATG G AAGAGAAAAATAAA

Sense strand PCR primer to prepare construct pET-pf JRTSΔ232-299. Bold-face letters represent Met introduced in front of Glu300. Underlined sequence is the restriction site for Nco I

N3TS

22

ACTCATGGATCC TTAAGCAGCC

Antisense strand PCR primer for the construction of all pET-pf JRTS mutants. Underlined sequence is the restriction site for BamH I

Back to article page

Malaria Journal

ISSN: 1475-2875

Contact us