Natural selection and recombination event in PvMSP-3β. (A) The sliding window plot of nucleotide diversity per site (π) was constructed to compare the level of genetic diversity in the N-terminal and C-terminal domains of PvMSP-3β. The π values were calculated using the DnaSP with a window length of 100 bp and step size of 25 bp. (B) The linkage disequilibrium (LD) plot showed non-random associations between the nucleotide variants in 95 Korean P. vivax isolates at different polymorphic sites. The R2 values were plotted against nucleotide distance using a two-tailed Fisher’s exact test for statistical significance.