Summary of the complete protocol. Plasmodium falciparum c ulture is maintained at 1% haematocrit during the first week to scale up the P. falciparum culture. Plasmodium falciparum culture is synchronized with 5% wt/v sorbitol and then adjusted to H = 0.8% and P = 2%. Percoll is carried out after three days to purify the schizonts and P. falciparum culture is adjusted to H = 1% and P = 1%. Forty-eight hours later, P. falciparum culture enriched with mature forms is passed through the vario mac magnetic column to isolate the schizonts-enriched iRBCs. iRBCs obtained are adjusted to H = 0.2% and P = 10% and after 18 hours P. falciparum ring-enriched culture at approximately 40% parasitaemia is harvested. If a trophozoite or schizont-enriched population is required, media has to be changed and P. falciparum culture will be harvested next day.