In vitro characterization of Pf HSBP. (A) 15% SDS-PAGE gel and immunoblot showing purified recombinant Pf HSBP. Lanes:- TL - total lysate, SP - pellet remaining after sonication, SS -supernatant after sonication, FT -flow through, 20 mM - wash with 20 mM imidazole, 50 mM - wash with 50 mM imidazole, and 200 mM - elution with 200 mM imidazole. (B) 2-D-electrophoresis of purified recombinant protein. (C) MS-based identification of Pf HSBP. (D) Circular dichroism spectrum of Pf HSBP.