In vivo Pf HSBP expression. (A) Pf HSBP exists in P. falciparum as a trimer and hexamer (Lanes 1 and 3 -P. falciparum lysate; lanes 2 and 4 - purified recombinant protein. Lanes 1 and 2 were probed with polyclonal antibody generated against Pf HSBP, whereas, lanes 3 and 4 were probed with pre-immune serum). (B) Size exclusion chromatography of Pf HSBP. Immunoblot shows the fractions of P. falciparum lysate containing Pf HSBP (top panel). The graph represents quantitation of Pf HSBP signal obtained (bottom panel). (C) Immunoprecipitation by Pf HSBP antiserum shows pull down of hexameric Pf HSBP, Lane 1-Protein-G control, Lane 2- Pre immune serum control, Lane 3- Pf HSBP IP. (D) Pf HSBP expression pattern in different blood stages of P. falciparum. (R - Ring stage, T - Trophozoite stage, S - Schizont stage). β-actin was used as a loading control. Bar diagram shows quantitative amounts of Pf HSBP present in different stages of P. falciparum.