Sequence alignments of amino acid sequences of 12 pvcsp distinct allelic variants and frequencies of pvcsp allelic variants identified from 45 P. vivax Anhui isolates. (A) Sequences were aligned to the amino acid sequences of the reference strain VK210 (M28746). Dots and dashes represent identical residues and deletions, respectively. Amino acid changes resulting from nucleotide substitutions are shown in bold. (B) Allelic frequencies of 12 pvcsp allelic variants obtained from P. vivax Anhui isolates.