Sporozoite immunization of human volunteers under chemoprophylaxis induces functional antibodies against pre-erythrocytic stages of Plasmodium falciparum

Behet, Marije C; Foquet, Lander; van Gemert, Geert-Jan; Bijker, Else M; Meuleman, Philip; Leroux-Roels, Geert; Hermsen, Cornelus C; Scholzen, Anja; Sauerwein, Robert W

doi:10.1186/1475-2875-13-136

Table 1 Reduction of liver parasite burden by CPS-induced antibodies

From: Sporozoite immunization of human volunteers under chemoprophylaxis induces functional antibodies against pre-erythrocytic stages of Plasmodium falciparum

	Mouse ID	IgG sample ID	Time point	Human albumin levels (mg/ml)	Proportion of HuHEP^bin mouse liver (%)	Weight mice (g)	Number of fed mosquitoes (out of 20)	Liver parasite burden (No. *Pf/* 10^6 HuHEP)	No. of Pf^cpositive liver sections (out of 12)
Pre-CPS^aIgG
Study 1	B730R	Pool 1	Pre	2,4	26,0	15,9	14	326,5	10
	K1596RL	Pool 2	Pre	2,6	37,2	11,5	19	209,3	5
	B749RL	Pool 3	Pre	3,0	39,7	12,1	17	77,2	5
	K1589R	Pool 4	Pre	4,0	33,0	12,5	14	280,1	11
	K1576	Pool 5	Pre	3,1	35,0	12,2	20	373,0	10
*Median*				3, 0	*35,* 0	*12,* 2	17	*280,* 1	10
Study 2	K1630LR	Vol.^d 1	Pre	3,0	40,1	15,1	16	354,5	7
	B934R	Vol. 2	Pre	2,2	29,1	11,7	18	329,8	4
	B934LR	Vol. 3	Pre	2,6	37,7	12,8	17	37,6	3
	B934L	Vol. 4	Pre	1,7	28,5	9,6	12	463,1	4
	K1575	Vol. 5	Pre	1,8	34,4	17,3	15	342,1	2
	B881R	Vol. 7	Pre	2,2	33,0	8,4	13	300,3	8
*Median*				2, 2	*33,* 7	*12,* 3	16	*336,* 0	4
*Median Pre-* *CPS IgG Study 1 and 2:*				2, 6	*34,* 4	*12,* 2	16	*326,* 5	5
Post-CPS IgG
Study 1	K1577L	Pool 1	Post	1,9	29,2	15	16	90,5	1
	K1540L	Pool 2	Post	2,6	31,6	12,6	18	13,1	3
	B733	Pool 3	Post	5,2	40,8	6,7	16	46,4	2
	K1589	Pool 4	Post	4,5	35,9	13,1	15	20,8	3
	B750L	Pool 5	Post	2,5	33,4	12,2	16	302,6	6
*Median*				2, 6	*33,* 4	*12,* 6	16	*46,* 4	3
Study 2	K1666L	Vol. 1	Post	4,6	41,0	15,7	14	37,1	3
	B934	Vol. 2	Post	1,8	17,4	11,7	16	6,7	1
	B905	Vol. 3	Post	4,2	41,2	12,7	18	28,3	2
	K1630L	Vol. 4	Post	1,7	21,2	14,6	14	60,9	2
	B865	Vol. 5	Post	2,3	36,5	17,3	14	29,2	3
	K1683	Vol. 7	Post	2,3	31,3	9,7	15	19,3	3
*Median*				2, 3	*33,* 9	*13,* 7	15	*28,* 8	3
*Median Post-CPS IgG Study 1 and 2:*				2, 5	*33,* 4	*12,* 7	16	*29,* 2	3
Significant difference Post- versus Pre-CPS IgG^e				ns	ns	ns	ns	p = 0.0008	p = 0.003

^aCPS ChemoProphylaxis and Sporozoites immunization.
^bHuHEP human hepatocytes.
^c Pf P. falciparum.
^dVol. volunteer.
^eStatistics: significant difference between Post- and Pre-CPS IgG calculated using the non-parametric Mann–Whitney U-test.

Back to article page

ISSN: 1475-2875

Contact us

Submission enquiries: journalsubmissions@springernature.com

Malaria Journal

Contact us