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Figure 2 | Malaria Journal

Figure 2

From: Molecular characterization of Plasmodium falciparum uracil-DNA glycosylase and its potential as a new anti-malarial drug target

Figure 2

Analysis of recombinant PfUDG expression by 12% SDS-PAGE. The gel was stained with Coomassie blue R250. Lane M, molecular weight markers; lane 1, non-induced whole cells of E. coli Rosetta expression host; lane 2, 0.5 mM IPTG-induced whole cells of E. coli Rosetta expression host; lane 3, non-induced whole cell of E. coli Rosetta carrying PfUDG-constructed vector; lane 4, 0.5 mM IPTG-induced whole cell of E. coli Rosetta carrying PfUDG-constructed vector; lane 5, total soluble protein of 0.5 mM IPTG-induced whole cell of E. coli Rosetta carrying PfUDG-constructed vector; lane 6, Ni-NTA agarose purified protein. The arrows indicate the completed (40 and 30 kDa) 6-residue histidine-tagged PfUDG.

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