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Table 4 Kinetic parameters of recombinant PfUDG

From: Molecular characterization of Plasmodium falciparum uracil-DNA glycosylase and its potential as a new anti-malarial drug target

Substrate Km(μM) kcat(min-1) kcat/Km(min-1/μM-1)
  PfUDG hUNG2* PfUDG hUNG2* PfUDG hUNG2*
ss-U 1.633 2.2 ± 0.2 59.6 2,768 ± 105 36.50 1,258
ds-A:U 1.656 0.7 ± 0.1 60.0 137 ± 4 36.23 187
  1. Each reaction was performed in 2 independent experiments in a standard reaction buffer containing 20 mM Tris–HCl, pH 8.0, 1 mM DTT, 1 mM EDTA, 50 mM NaCl, 100 μg/ml BSA, 2.5 nM recombinant PfUDG, and 0.01-3.5 μM 32P-labelled single strand oligo-U (ss-U) or double stranded A:U (ds-A:U) at 37°C for 10 min.
  2. *[27].