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Table 1 Detection characteristics of MMSR assay in comparison to “wet” assay

From: Sample-ready multiplex qPCR assay for detection of malaria

  MMSR assay Wet assay Parasite/μL
CTMean CTSD CTMean CTSD
PLU assay 18.55 0.013 19.78 0.191 78240
FAL assay 20.07 0.031 20.85 0.055  
RNaseP assay 24.13 0.083 30.09 0.081  
PLU assay 20.73 0.092 22.17 0.227 15648
FAL assay 22.23 0.054 23.62 0.338  
RNaseP assay 24.52 0.185 29.57 0.082  
PLU assay 23.58 0.029 24.89 0.616 3129
FAL assay 24.93 0.001 26.06 0.029  
RNaseP assay 25.37 0.055 29.43 0.492  
  1. NIBSC standard DNA was five-fold serially diluted times. The performance of MMSR assay was compared to the “wet” assay. The performance of the MMSR assay was comparable to that of the “wet” assay for the three assay targets tested.