The effect of gonotrophic cycle length and number of pre-infectious cycles on relative lifetime number of infectious bites per female resulting from manipulation. In both graphs, the values reported are based on transmission site data, each line represents a location (associated daily mortality value) with an even split between daily and feeding-associated mortality. One value was held constant while the other was varied (Tables 1 and 2) The relative lifetime number of infectious bites reported is for a scenario in which all manipulated females skip pre-infectious feeding cycles (M = 1) and they take five bites per infectious feeding attempt (A = 5). A. the number of pre-infectious cycles was held constant and the duration of gonotrophic cycles was altered. B. Effect of the number of pre-infectious cycles by holding the cycle duration constant and varying the number of pre-infectious cycles.