Skip to main content
Figure 7 | Malaria Journal

Figure 7

From: Glomerular changes and alterations of zonula occludens-1 in the kidneys of Plasmodium falciparum malaria patients

Figure 7

ZO-1 expression in cell junctions of EC-PRBC co-culture. Confluent EC monolayers were exposed to media alone (control), RBCs, or PRBCs for 24 hrs. After incubation, the ECs were stained with primary antibodies directed against ZO-1, followed by Alexafluor 488-conjugated secondary antibodies. Intact tight junction-associated protein is shown in normal ECs (incubated with medium alone) (Panel A) and ECs incubated with RBCs (Panel B). Both panels (A, B) display continuity of ZO-1 expression along the cell membrane with no alteration (arrows). Damaged tight-junction-associated protein, ZO-1, is seen in ECs incubated with PRBCs (Panel C) (asterisks) (1,000x). Green- ZO-1, Blue- nuclei.

Back to article page